Prokaryotic expression, purification, polyclonal antibody preparation, and tissue distribution of porcine Six1

Prokaryotic expression, purification, polyclonal antibody preparation, and tissue distribution of porcine Six1

Prokaryotic Expression, Purification, and Polyclonal Antibody Production of a Truncated Recombinant Rabies Virus L Protein

Background: Rabies virus (RABV) is a deadly neurotropic virus that causes the disease of rabies in humans and animals. L protein is one of the large structural protein of rabies virus, which displays multiple enzymatic activities, and is required for viral transcription and replication. Objectives: A truncated L protein of Rabies virus is being cloned, expressed and purified to produce relevant...

Parathyroid hormone-related peptide (PTHrP): prokaryotic expression, purification, and preparation of a polyclonal antibody.

Parathyroid hormone-related peptide (PTHrP) plays important roles in promoting cancer occurrence and in the development of bone metastases. To increase our knowledge of the biological functions of PTHrP, the prokaryotic expression vector pET-PTHrP was successfully constructed and the His-PTHrP fusion protein was expressed in Escherichia coli. Anti-PTHrP polyclonal antibody was then prepared fro...

Prokaryotic expression and purification of porcine Sox6

Wanxue WEN*, Xiaoling CHEN*, Zhiqing HUANG**, Meng XU, Daiwen CHEN, Bing YU, Jun HE, Junqiu LUO, Xiangbing MAO, Jie YU, Ping ZHENG, Hong CHEN Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China College of Food Science, Sichuan Agricultural University, Yaan, Sichuan, P....

prokaryotic expression, purification, and polyclonal antibody production of a truncated recombinant rabies virus l protein

background: rabies virus (rabv) is a deadly neurotropic virus that causes the disease of rabies in humans and animals. l protein is one of the large structural protein of rabies virus, which displays multiple enzymatic activities, and is required for viral transcription and replication.objectives: a truncated l protein of rabies virus is being cloned, expressed and purified to produce relevant ...